Spectral unmixing explorer
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Download mixing matrix
Scale fluorophore spectra by brightness
Primary dichroic / notch filter
Spectrally flat (e.g. 80:20 beamsplitter)
Spectrally flat (e.g. 80:20 beamsplitter)
Detector set
PRISM 458/491/514/532/561/594/633
PRISM 458/491/514/532/561/594/633
Letter brightness
1
1,000
0
0
100
1
101
201
301
401
501
601
701
801
901
1,000
Fluorophore 1
TagBFP
TagBFP
Fluorophore 2
Cerulean
Cerulean
Fluorophore 3
mAzamiGreen
mAzamiGreen
Fluorophore 4
Citrine
Citrine
Fluorophore 5
mCherry
mCherry
Fluorophore 6
iRFP670
iRFP670
Fluorophore 7
**None**
**None**
Fluorophore 8
**None**
**None**
Dichroic spectra
Fluorophore spectra
Mixing matrix
Channel spectra
Detector spectra
Ground truth image
Mixed image
Linearly unmixed image
Phasor plot
CRLB analysis
Object 1 expected count
0
1,000
0
0
100
0
100
200
300
400
500
600
700
800
900
1,000
Object 2 expected count
0
1,000
0
0
100
0
100
200
300
400
500
600
700
800
900
1,000
Object 3 expected count
0
1,000
0
0
100
0
100
200
300
400
500
600
700
800
900
1,000
Object 4 expected count
0
1,000
0
0
100
0
100
200
300
400
500
600
700
800
900
1,000
Object 5 expected count
0
1,000
0
0
100
0
100
200
300
400
500
600
700
800
900
1,000
Object 6 expected count
0
1,000
0
0
100
0
100
200
300
400
500
600
700
800
900
1,000
Object 7 expected count
0
1,000
0
0
100
0
100
200
300
400
500
600
700
800
900
1,000
Object 8 expected count
0
1,000
0
0
100
0
100
200
300
400
500
600
700
800
900
1,000
single channel detection
,
user-selected detection
,
infinitesimal binning detection
(solid line = no photon loss, dashed line = with primary dichroic photon loss)