Spectral unmixing explorer

Download mixing matrix

Scale fluorophore spectra by brightness

Primary dichroic / notch filter

Detector set

Letter brightness

Fluorophore 1

Fluorophore 2

Fluorophore 3

Fluorophore 4

Fluorophore 5

Fluorophore 6

Fluorophore 7

Fluorophore 8

Dichroic spectra

Fluorophore spectra

Mixing matrix

Channel spectra

Detector spectra

Ground truth image

Mixed image

Linearly unmixed image

Phasor plot

CRLB analysis

Object 1 expected count

Object 2 expected count

Object 3 expected count

Object 4 expected count

Object 5 expected count

Object 6 expected count

Object 7 expected count

Object 8 expected count

single channel detection, user-selected detection, infinitesimal binning detection
(solid line = no photon loss, dashed line = with primary dichroic photon loss)